Part:BBa_K277113

3L.3_23.D2.09

3L.3_23.D2.09 is 752 bases long and is cloned into the pGem-T vector.

3L.3_23.D2.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D2.09 is a constituent of 3L.3_23.D2 (along with 3L.3_23.D2.01, 3L.3_23.D2.02, 3L.3_23.D2.03, 3L.3_23.D2.04, 3L.3_23.D2.05, 3L.3_23.D2.06, 3L.3_23.D2.07, 3L.3_23.D2.08, 3L.3_23.D2.10, 3L.3_23.D2.11, 3L.3_23.D2.12, 3L.3_23.D2.13, 3L.3_23.D2.14, 3L.3_23.D2.15, 3L.3_23.D2.16, and 3L.3_23.D2.17.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

loxP_site loxPsym_YCL012C (72..105)

gene YCL012C (109..513) Putative protein of unknown function%3B orthologs are present in S. bayanus%2C S. paradoxus and Ashbya gossypii%3B YCL012C is not an essential gene

Sequence (corresponds to coordinates 82203..82954 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 328